Cryptococcus neoformans var grubii C.neoformans molecular
typing articles listed in Pubmed
Cryptococcus neoformane RSS Genotype and mating type analysis of Cryptococcus neoformans and Cryptococcus gattii isolates from China that mainly originated from non-HIV-infected patients. Feng X, Yao Z, Ren D, Liao W, Wu J FEMS Yeast Res : PubMed:18671745 Click here to toggle abstract
Genotype and mating type analysis of Cryptococcus neoformans and Cryptococcus gattii isolates from China that mainly originated from non-HIV-infected patients.
FEMS Yeast Res. 2008 Sep;8(6):930-8
Authors: Feng X, Yao Z, Ren D, Liao W, Wu J
Cryptococcosis has been reported to be mostly associated with non-HIV-related patients in China. However, little is known about the molecular characteristics of clinical isolates from the Cryptococcus neoformans species complex in this country. In this study, 115 clinical isolates were included. Molecular type VNI was the most representative (n=103), followed by VGI (n=8), VNIII (n=2), VNIV (n=1), and VGII (n=1). With the exception of a serotype D mating type a isolate, all possessed the MATalpha locus. Multilocus sequence typing (MLST) revealed that most Cryptococcus gattii isolates from China shared identical MLST profiles with the most common MLST genotype reported in the VGI group, and the only one VGII isolate resembled the Vancouver Island outbreak minor genotype. The C. gattii strains involved in this study were successfully grouped according to their molecular type and mating types by PCR-restriction fragment length polymorphism (RFLP) analysis of the GEF1 gene. Our results suggest that (1) in China, cryptococcosis is mostly caused by C. neoformans var. grubii (molecular type VNI), and mating type alpha; (2) The most common causative agents of C. gattii infection in China are closely related to a widely distributed MLST genotype; and (3) The PCR-RFLP analysis of the GEF1 gene has the potential to identify the molecular and mating types of C. gattii simultaneously.
PMID: 18671745 [PubMed - indexed for MEDLINE]
Association between fertility and molecular sub-type of global isolates of Cryptococcus gattii molecular type VGII. Ngamskulrungroj P, Sorrell TC, Chindamporn A, Chaiprasert A, Poonwan N, Meyer W Med Mycol : PubMed:18651305 Click here to toggle abstract
Association between fertility and molecular sub-type of global isolates of Cryptococcus gattii molecular type VGII.
Med Mycol. 2008 Nov;46(7):665-73
Authors: Ngamskulrungroj P, Sorrell TC, Chindamporn A, Chaiprasert A, Poonwan N, Meyer W
The basidiomycetous yeast Cryptococcus gattii, is a primary pathogen which causes disease in apparently healthy humans and a wide range of animals. Recently, an outbreak of cryptococcosis caused by a previously uncommon genotype of C. gattii, VGII, emerged on Vancouver Island, British Columbia, Canada. Two pathogenic sub-types of VGII (designated VGIIa and VGIIb) were identified among these isolates. All of the isolates proved to be mating type alpha and had exceptionally high sporulation capacity. The common subtype, VGIIa, was more virulent than VGIIb in mice, suggesting a linkage between subtype and fertility/virulence. To test this hypothesis, we compared the fertility of 91 isolates from the Vancouver Island outbreak with that of 72 VGII isolates selected globally. Of all isolates, 69.94% were found to be fertile and exhibited clamp connections and basidiospores. The Vancouver isolates showed a high fertility rate of 84.2% as compared to only 29% of the 21 Australian isolates investigated. Mating type alpha strains were more fertile (72.79%) than mating type a (43.75%) (p<0.022). Amongst the two subtypes of VGII a much higher proportion of VGIIa (91.7%) than VGIIb (33.3%) was fertile (p<0.001). These results suggest that there is a clear correlation between the VGII subtypes of C. gattii and their mating/fertility. Further in vitro and in vivo investigations of more strains and congenic pairs are warranted.
PMID: 18651305 [PubMed - indexed for MEDLINE]
Multilocus microsatellite typing for Cryptococcus neoformans var. grubii. Hanafy A, Kaocharoen S, Jover-Botella A, Katsu M, Iida S, Kogure T, Gonoi T, Mikami Y, Meyer W Med Mycol : PubMed:18608927 Click here to toggle abstract
Multilocus microsatellite typing for Cryptococcus neoformans var. grubii.
Med Mycol. 2008 Nov;46(7):685-96
Authors: Hanafy A, Kaocharoen S, Jover-Botella A, Katsu M, Iida S, Kogure T, Gonoi T, Mikami Y, Meyer W
Fifteen randomly selected microsatellites (simple sequence repeats; SSRs), from the H99 Cryptococcus neoformans var. grubii (serotype A) genome, were sequenced, characterized and applied to sequence 87 clinical and environmental C. neoformans var. grubii isolates from 12 different countries based on Multilocus Microsatellite Typing (MLMT). Among the 15 SSR loci, three (designated CNG1, CNG2 and CNG3) were polymorphic, while the remaining 12 SSR loci showed no variations. The specific PCR primers of the polymorphic microsatellites, i.e., CNG1, CNG2 and CNG3, amplified those loci only from strains of C. neoformans (C. neoformans var. grubii, C. neoformans var. neoformans and the AD hybrid) but not from Cryptococcus gattii, suggesting a species-specific association. The three polymorphic microsatellites are useful markers for strain genotyping, population genetic analysis, epidemiological studies, and may be helpful for the diagnosis of cryptococcosis due to C. neoformans.
PMID: 18608927 [PubMed - indexed for MEDLINE]
